Hello all,
Posting this on behalf of Dr. Greg Holland from San Diego State University.
The full posting is shown below as well as attached in pdf format.
Best,
David O.
David Onofrei, Ph.D.
NMR Facility Director
San Diego State University
Department of Chemistry
Email: chem-nmr_at_sdsu.edu<mailto:chem-nmr_at_sdsu.edu>
Holland Lab Postdoctoral Position in Natural Soft Matter, Dept. of Chemistry, SDSU
A DOD-funded postdoctoral position is available at San Diego State University
in the general area of exploring atomic and molecular level structure and dynamics
in spider silk proteins with solution and solid-state NMR together with cryo-TEM
and molecular dynamics (MD) simulations. Developing a fundamental understanding of
how spider silk proteins assemble into high performance biomaterials will impact
applications in the defense, medical and space exploration sectors. The biochemical
transformation processes that take place to synthesize protein-based materials
remains a complicated problem with numerous unanswered questions. These structural
evolutions are mostly unexplored due to the lack of combined experimental data that
can link the atomic, molecular and meso- length scales. The aim of this research is
to harness recent advances in magnetic resonance, cryo-TEM and simulation to develop
models for the transformation of soluble silk proteins to materials that out-perform
man-made systems.
The successful applicant will become part of a large multi-university team to
investigate and characterize spider silk structural biology from solution through the
solid, fibrous material. The applicant must possess outstanding communication skills
and be able to clearly communicate the scientific significance of this work to colleagues
with backgrounds in theory, chemistry, biology and materials science. The applicant will
be responsible for acquiring and analyzing multi-dimensional, multinuclear solution and
magic angle spinning (MAS) solid-state NMR data of spider silk proteins. NMR data will be
combined with data from cryo-TEM imaging and tomography and MD simulations to begin
connecting the atomic, molecular and nanometer length scales for an improved understanding
of hierarchical silk formation. Extensive image simulation with dynamic models provided by
computation will be integrated into the data analysis and interpretation.
Basic Qualifications:
* A Ph.D. in physics, chemistry, materials science or related disciplines.
* Experience in solution and MAS solid-state NMR of proteins.
* Experience in analyzing NMR data with TOPSPIN, NMRPipe, Sparky, TALOS-N, CS-ROSETTA, etc.
* Experience in MATLAB programming to combine and analyze data sets from multiple techniques.
Preferred Qualifications:
* Experience in cryo-TEM tomography of protein oligomers.
* Experience in MD simulations of proteins.
Contact gholland_at_sdsu.edu<mailto:gholland_at_sdsu.edu> for more info.
Received on Tue Feb 09 2021 - 15:18:41 MST