There is a student here doing a ROESY buildup study and I'm
wondering whether we should use the raw crosspeak volumes or normalize them
by dividing by the volume of a diagonal peak or some "standard" cross-peak?
Do you need to correct for the T1(rho) decay which occurs during the
spin-lock? The goal is to determine initial buildup rates for distance
calculation and to distinguish direct ROEs from spin diffusion in a peptide
(in DMSO). The study is complicated by the fact that we used different
receiver gains (Bruker RG) for different mixing times (oops!).
Neil E. Jacobsen
NMR Facility Manager
Department of Chemistry
University of Arizona
Tucson, AZ 85721
520-621-8146
Fax 520-621-8407