Re: BB Probe for Perfusion Exp'ts

Bruce D. Ray (bray@indyvax.iupui.edu)
Thu, 12 Jan 1995 10:02:09 +0000

>Dear Folks:
>
> Someone asked me for information about hign resolution BB probe
>for 'perfusion' experiments, unfortunately I did not have anything to
>offer. Does anyone know (1) who can make such probe,

Dr. Craig Bradley
Cryomagnet Systems
4101 Cashard Ave.
Indianapolis, IN 46203
USA

> (2) whether some
>modifications have to be done to Bruker 89mm MSL300 with high resolution
>capacity,

Probably not, but talk to Dr. Bradley for confirmation on this for
your sopecific machine.

>(3) How much the probe will cost, and

Somewheres in the neighborhood of $11,000 to $13,000 depending on
frequencies desired, temperature control desired, etc. Most likely
it will be about $12,000. Here, again, Dr. Bradley can give you a better
idea of costs.

>(4) is that possible to
>use a regular BB probe modified with interchangeable parts?

Well, I've collaborated with our Medical Physiology Department on
perfusion of dog pulmonary artery without any special probe or
equipment. We used a standard 10 mm NMR tube and unmodified,
10 mm BB probe with tubing to provide gravity flow input of perfusate
with active pump out using a multi-channel peristaltic pump and
multiple tubes {with backups} to pump out perfusate. We used a valve
on the inlet side to regulate flow and oxygenated by defusing 95%
oxygen/5% carbon dioxide into the ingoing perfusate as it flowed
through a heated glass Allihn condensor. A larger capacity condensor,
or perfusion lung might work better.

> Anything
>related to 'NMR perfusion experiemnts' is highly wellcome. Thanks.

Hope this is helpful.

Sincerely,

--
Bruce D. Ray
bray@indyvax.iupui.edu
Operations Director
NMR Center
Physics Dept.
IUPUI
402 N. Blackford St.
Indianapolis, IN  46202-3273

voice: 317-274-6914 fax: 317-274-2393