Hi George,
TopSpin does give absolute values, go to the other integration menu (how to
get there depends on what TopSpin version and what the set up is, often it
is in the menu bar). If you read the spectra into MNova, the absolute values
can also be viewed. But this simplest way to do what you want is to integrate
the signal of choice in one spectrum, calibrate to what you want (e.g., 100%)
and then exit the integration with saving. In the next spectrum, integrate
the signal and right click and choose "use last calibration".
Best,
Karel
________________________________________
From: Sukenick, George <g-sukenick_at_ski.mskcc.org>
Sent: 18 October 2023 02:01
To: main_at_ammrl.groups.io
Subject: [AMMRL] integration between spectra
I have a researcher who wants to compare integration between different spectra
(all taken with same parameters).
My thought was to integrate without normalizing or referencing the integral,
but it seems that integration (at least in Topspin) does not provide the
absolute value.
I don’t see anything in the AU program collection either.
Is there a way to obtain this information?
Or while we always use relative integrals – is there a reason not to use
the absolute values?
I suppose that the data could be imported to a spreadsheet and then absolute
intensities summed within a region, but there should be a better way?
Thanks!
George Sukenick, Ph.D.
Associate Lab Member/Head
NMR Analytical Core Facility
Sloan Kettering Institute
Memorial Sloan Kettering Cancer Center
g-sukenick_at_ski.mskcc.org<mailto:g-sukenick_at_ski.mskcc.org>
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Received on Tue Oct 17 2023 - 18:50:28 MST