We have used MDPA for years in our perfusion studies with the eye. It
was sealed in a capillary and put directly in the perfusion cell. In
our calibration studies with phosphoric acid set to 0.0ppm, MDPA was
referenced to 18.0ppm, out of the way of anything of interest. Good
luck.
Dwaine.
Dwaine Thomas
NMR Spectroscopy
Alcon Labs, Inc.
M/S: R2-19
6201 South Freeway
Fort Worth, TX 76134
Phone: 817-551-8003
FAX: 817-551-4584
email: dwaine.thomas@alconlabs.com
>----------
>From: Edward T. Chainani[SMTP:etch@ginto.chem.admu.edu.ph]
>Sent: Wednesday, May 20, 1998 5:04 AM
>To: AMMRL
>Subject: 31P reference
>
>ammrl'ers,
>
>does anybody know of a reference compound for 31P NMR that doesn't crowd
>in with the other peaks in biological systems? The typical reference
>compound, 85% phosphoric acid, has a peak in the middle of the chemical
>shift range of interest.
>
>I've encountered methylenediphosphonic acid (CH6O6P2) in the literature
>(Magnetic Resonance in Medicine 33:795-800; 1995). Has anyone ever used
>this? I hope someone can tell me the chemical shift with respect to
>phosphoric acid.
>
>-----------------------------------------------------------------------------
>-
>Edward T. Chainani Nuclear Magnetic Resonance Laboratory Manager
>
> National Chemistry Instrumentation Center (NCIC)
>
>Ateneo de Manila University phone: (632) 426-6001 loc. 2309
>Loyola Heights, Quezon City e-mail: etch@ginto.chem.admu.edu.ph
>Philippines FAX: (632) 426-5985
>-----------------------------------------------------------------------------
>-
>
>
>