Dear Colleagues,
I am posting this for Dr. Unthank. Please respond to him directly
(Dr. Joseph L. Unthank <junthank_at_iupui.edu>)
Dr. Unthank's work focuses on mechanisms of collateral artery
development. He uses the rat mesentery as a model. He got the suggestion
that he must use electron magnetic resonance to quantitate superoxide
levels in an in vivo setting. Is this feasible? Can any one assist with
this?
For his application, he would need to quantify reactive oxygen species
with changes in flow in vivo (rat mesentery and ileal arteries could be
exteriorized and placed in an organ support chamber while still
attached to the animal for perfusion). These vessels are much smaller
than the rat and rabbit aorta. Each artery weighs less than 0.5mg.
Please advise him regarding the feasibility of such measurements. If
you believe it might be possible, the first thing to try would probably
be to measure production before and after superoxide
scavengers/inhibitors.
Ulrike Werner-Zwanziger
--
Ulrike Werner-Zwanziger
Dep. of Chemistry Tel.: (812) 856-4629
Indiana University Fax.: (812) 855-8300
800 E. Kirkwood Ave
Bloomington, IN 47405
Received on Fri Mar 08 2002 - 18:52:49 MST