Re: [AMMRL] 1.7mm NMR tubes contaminants

From: johnhahn cazent.com <johnhahn_at_cazent.com>
Date: Fri, 21 Jul 2023 13:41:22 +0000

 Hi Genevieve,

 Let me follow up on my previous comment concerning sample contamination...
I was referring to the pipette tubes or whatever you are using to transfer
the sample to the 1.7mm NMR tube. The NMR sample tubes themselves are generally
good...although assumptions always tend to get ME in trouble... Clemens is
spot on...if we had a look at the data, we could get a handle on what
the contamination is...
[cid:a17c86e9-ccaa-4b53-9cb7-17ee38253ae2]

Regards,
John E. Hahn

CAZ Enterprises, LLC
6320 Fly Rd. Ste 207
East Syracuse, NY 13057
Ph. 315-727-8192
http://www.Cazent.com

________________________________
From: main_at_ammrl.groups.io <main_at_ammrl.groups.io> on behalf of Akien, Geoffrey via groups.io <g.akien=lancaster.ac.uk_at_groups.io>
Sent: Friday, July 21, 2023 3:10
To: main_at_ammrl.groups.io <main_at_ammrl.groups.io>
Subject: Re: [AMMRL] 1.7mm NMR tubes contaminants

Hi Genevieve,

It's not necessarily something you did or didn't do - all solvents contain
impurities if you look hard enough:
https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/mrc.4410

Thanks

Geoff
________________________________
From: main_at_ammrl.groups.io <main_at_ammrl.groups.io> on behalf of johnhahn cazent.com <johnhahn_at_cazent.com>
Sent: Friday, July 21, 2023 03:09
To: main_at_ammrl.groups.io <main_at_ammrl.groups.io>
Subject: [External] Re: [AMMRL] 1.7mm NMR tubes contaminants



Hi Genevieve,

One must be careful with sample prep and be sure that the tubes are clean.
A lot of times, depending on the supplier, the tubes are coated with oil to
protect them. They need to be cleaned thoroughly before use. This would be
but one possible source. Regards, John \\\


________________________________
From: main_at_ammrl.groups.io <main_at_ammrl.groups.io> on behalf of gseabroo via groups.io <gseabroo=uhnresearch.ca_at_groups.io>
Sent: Thursday, July 20, 2023 6:40:20 PM
To: main_at_ammrl.groups.io <main_at_ammrl.groups.io>
Subject: [AMMRL] 1.7mm NMR tubes contaminants


Hello all,
I hope you are enjoying your summer.

I'm running into a roadblock. I would like to see if I can detect very low
concentration (uM range) of butyrate in plasma using my Bruker 600MHz
spectrometer equipped with a TCI 1.7mm micro-cryoprobe. Therefore I have to
use the 1.7mm NMR tubes. However, when I ran my 100uM Butyrate control sample
I noticed way more peaks than expected. See attached file. I decided to check
first the D2O. I used two different lots of D2O from one company and one lot
from another company. I still see the same extra peaks. Therefore, the extra
peaks have to come from the 1.7mm tubes. Has anyone seen this before? If yes,
how did you deal with these extra peaks and 1.7mm tubes handling?
Thanks in advance for any advices and inputs! Greatly appreciated.

Best,
Geneviève

--

Dr. Geneviève Seabrook
Scientist, UHN-Princess Margaret Cancer Centre
NMR Research & NMR Core Facility Manager
Toronto, Canada
Email : Genevieve.Seabrook_at_uhnresearch.ca<mailto:Genevieve.Seabrook_at_uhnrese=
arch.ca>
Mobile: 416-629-7391
https://nmrcore.uhnresearch.ca/




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Received on Fri Jul 21 2023 - 06:56:36 MST