Hi Genevieve
this might be a contamination from the packaging of the tubes. It has
happened before that the manufacturer has switched the material and
something like this was the result. A few questions when did you receive
the tubes? Were the racks packed in a plastic wrapper? If the rack or
packaging had a batch number even better.
Do you get a clean spectrum after one rinse with D2O?
And can you please send me the data file of one of your spectra.
Thanks
Clemens
On 7/20/2023 6:40 PM, gseabroo_at_uhnresearch.ca<mailto:gseabroo_at_uhnresearch.ca> wrote:
Hello all,
I hope you are enjoying your summer.
I'm running into a roadblock. I would like to see if I can detect very low
concentration (uM range) of butyrate in plasma using my Bruker 600MHz
spectrometer equipped with a TCI 1.7mm micro-cryoprobe. Therefore I have to
use the 1.7mm NMR tubes. However, when I ran my 100uM Butyrate control sample
I noticed way more peaks than expected. See attached file. I decided to check
first the D2O. I used two different lots of D2O from one company and one
lot from another company. I still see the same extra peaks. Therefore, the
extra peaks have to come from the 1.7mm tubes. Has anyone seen this before?
If yes, how did you deal with these extra peaks and 1.7mm tubes handling?
Thanks in advance for any advices and inputs! Greatly appreciated.
Best,
Geneviève
--
Dr. Geneviève Seabrook
Scientist, UHN-Princess Margaret Cancer Centre
NMR Research & NMR Core Facility Manager
Toronto, Canada
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--
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