AMMRL: F2 splitting in HSQC

From: Lavoie, Serge <serge.lavoie_at_biology.gatech.edu>
Date: Sun, 17 Jan 2016 15:59:44 +0000

Dear AMMRL

I have acquired a 1H-13C HSQC spectra using the hsqcetgpsi2 pulse program with a natural product isolated from a marine organism. Almost all correlations look pretty good, but some are splitted in the F2 dimension. For example, a 1H singlet signal, as observed on the 1D 1H spectrum at δH 3.99, appear as a doublet on the HSQC (J = 47 Hz, δC 32.0). On the 1D 13C spectrum (1H decoupled), this carbon signal appear as a singlet. In many marine natural products, halogen atoms can be found. Do someone ever observed this phenomena? How it can be explained?

Thank you for your help


Serge Lavoie, PhD
Georgia Institute of Technology
Received on Sun Jan 17 2016 - 05:59:34 MST