Re: AMMRL: PO-WaterLOGSY

From: Zhang, Weixing <Weixing.Zhang_at_STJUDE.ORG>
Date: Tue, 13 Feb 2018 13:53:45 +0000

Dear All,

Thank everybody who spent your precious time to read my email.
Almost everybody think this is a lock-phase or lock stability issue, but I do not have this problem when I use the other version of waterLOGSY published by Dalvit in 2001.
That version uses a 180 degree pulse in the middle of the mixing time.

Here is another picture showing the spectra of the two separate scans when water is at +Z and –Z. Your comments are appreciated!

Thanks,
Weixing


[cid:e758d52a-2b47-419b-9877-76f7c73e4a45]


________________________________
From: Zhang, Weixing <Weixing.Zhang_at_STJUDE.ORG>
Sent: Sunday, February 11, 2018 8:16 PM
To: Ammrl
Subject: AMMRL: PO-WaterLOGSY


This sender failed our fraud detection checks and may not be who they appear to be. Learn about spoofing<http://aka.ms/LearnAboutSpoofing>
        Feedback<http://aka.ms/SafetyTipsFeedback>

Hello:

I am trying to run the PO-waterLOGSY experiment (AV-II, TCI, TopSpin 3.2), but I see a glitch on the left side of each peak (0.5 mM DSS, d1=3 sec, mix=0.8 or 1.2 sec). When I tried to collect the data of scan 1 and scan 2 separately, I found that the peak position of scan 1 shifted to the left side a little which is the cause of the glitch after the two scans were added together.

 [cid:38e940ae-5a6f-4735-8a2e-afde99946c45]

Has anybody seen this problem and how to fix it?

 Thank you in advance!



Weixing Zhang

 ----------Reference ---------------------------------------------------------------------------------

J Biomol NMR.<https://na01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F19205897&data=01%7C01%7CWeixing.Zhang%40stjude.org%7C99a3b0105f6d48fef74208d5725b7ec4%7C22340fa892264871b677d3b3e377af72%7C0&sdata=wuMUIJJQPnoEdQDAcQUzjOzM5Rl8wqZFmQ5C%2BJCU0p4%3D&reserved=0> 2009 Apr;43(4):211-7. doi: 10.1007/s10858-009-9303-5. Epub 2009 Feb 11.

Time efficient detection of protein-ligand interactions with the polarization optimized PO-WaterLOGSY NMR experiment.

Gossert AD<https://na01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F%3Fterm%3DGossert%2520AD%255BAuthor%255D%26cauthor%3Dtrue%26cauthor_uid%3D19205897&data=01%7C01%7CWeixing.Zhang%40stjude.org%7C99a3b0105f6d48fef74208d5725b7ec4%7C22340fa892264871b677d3b3e377af72%7C0&sdata=SQpx%2FJk%2F7py5PlwKtMkckE%2FUq3SPGrO5bMqrmfFE8RE%3D&reserved=0>1, Henry C<https://na01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F%3Fterm%3DHenry%2520C%255BAuthor%255D%26cauthor%3Dtrue%26cauthor_uid%3D19205897&data=01%7C01%7CWeixing.Zhang%40stjude.org%7C99a3b0105f6d48fef74208d5725b7ec4%7C22340fa892264871b677d3b3e377af72%7C0&sdata=ojgyoGcHiDx7t%2BdlVcnfaw6xwruZByA2bxTYUQqFMZ4%3D&reserved=0>, Blommers MJ<https://na01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F%3Fterm%3DBlommers%2520MJ%255BAuthor%255D%26cauthor%3Dtrue%26cauthor_uid%3D19205897&data=01%7C01%7CWeixing.Zhang%40stjude.org%7C99a3b0105f6d48fef74208d5725b7ec4%7C22340fa892264871b677d3b3e377af72%7C0&sdata=4XT9f8LH8F893QuQS0tWNyu12MedS8ENNws0yUpUmyA%3D&reserved=0>, Jahnke W<https://na01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F%3Fterm%3DJahnke%2520W%255BAuthor%255D%26cauthor%3Dtrue%26cauthor_uid%3D19205897&data=01%7C01%7CWeixing.Zhang%40stjude.org%7C99a3b0105f6d48fef74208d5725b7ec4%7C22340fa892264871b677d3b3e377af72%7C0&sdata=2h9RUYMTi8l9s7gAwjbozPonB0ab4MaYUz8ft632NOw%3D&reserved=0>, Fernández C<https://na01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F%3Fterm%3DFern%25C3%25A1ndez%2520C%255BAuthor%255D%26cauthor%3Dtrue%26cauthor_uid%3D19205897&data=01%7C01%7CWeixing.Zhang%40stjude.org%7C99a3b0105f6d48fef74208d5725b7ec4%7C22340fa892264871b677d3b3e377af72%7C0&sdata=Y1urXYolb014QESq32gqhpN%2FIsN%2FYTDCrtRxz46ebeM%3D&reserved=0>.




________________________________

Email Disclaimer: www.stjude.org/emaildisclaimer
Consultation Disclaimer: www.stjude.org/consultationdisclaimer

Received on Tue Feb 13 2018 - 03:53:56 MST