Date: Mon, 12 Feb 2018 02:16:27 +0000
Hello:
I am trying to run the PO-waterLOGSY experiment (AV-II, TCI, TopSpin 3.2), but I see a glitch on the left side of each peak (0.5 mM DSS, d1=3 sec, mix=0.8 or 1.2 sec). When I tried to collect the data of scan 1 and scan 2 separately, I found that the peak position of scan 1 shifted to the left side a little which is the cause of the glitch after the two scans were added together.
[cid:38e940ae-5a6f-4735-8a2e-afde99946c45]
Has anybody seen this problem and how to fix it?
Thank you in advance!
Weixing Zhang
----------Reference ---------------------------------------------------------------------------------
J Biomol NMR.<https://www.ncbi.nlm.nih.gov/pubmed/19205897> 2009 Apr;43(4):211-7. doi: 10.1007/s10858-009-9303-5. Epub 2009 Feb 11.
Time efficient detection of protein-ligand interactions with the polarization optimized PO-WaterLOGSY NMR experiment.
Gossert AD<https://www.ncbi.nlm.nih.gov/pubmed/?term=Gossert%20AD%5BAuthor%5D&cauthor=true&cauthor_uid=19205897>1, Henry C<https://www.ncbi.nlm.nih.gov/pubmed/?term=Henry%20C%5BAuthor%5D&cauthor=true&cauthor_uid=19205897>, Blommers MJ<https://www.ncbi.nlm.nih.gov/pubmed/?term=Blommers%20MJ%5BAuthor%5D&cauthor=true&cauthor_uid=19205897>, Jahnke W<https://www.ncbi.nlm.nih.gov/pubmed/?term=Jahnke%20W%5BAuthor%5D&cauthor=true&cauthor_uid=19205897>, Fernández C<https://www.ncbi.nlm.nih.gov/pubmed/?term=Fern%C3%A1ndez%20C%5BAuthor%5D&cauthor=true&cauthor_uid=19205897>.
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