We use integration of resonances from all nuclei for quantitation (including H, C, P, and F). You need to collect data with NOE suppressed and with relaxation delay of ca. 5 x T1. Routine carbon spectra are not usually collected under these conditions; they are usually collected with short relaxation delay and continuous proton decoupling (which produces NOE enhancement of signals). Also you need to pay attention to processing parameters (especially those that influence digital resolution) as these will affect the accuracy of integrals.
Peter L. Rinaldi
Emeritus Professor of Chemistry
Department of Chemistry
190 East Buchtel Commons
The University of Akron
Akron, Ohio 44325-3601
Telephone: 330-972-5990
Fax: 330-972-5256
Email: PeterRinaldi_at_uakron.edu
-----Original Message-----
From: Grace, David (GE Healthcare) [mailto:David.Grace_at_ge.com]
Sent: Friday, December 06, 2013 1:12 AM
To: Serkova, Natalie; bbanks_at_pharmacore.com; ammrl_at_ammrl.org
Subject: RE: AMMRL: integration of various nuclei
Hi
We use integration of 19F regularly in quantitative situations with good results especially if the resonances are closs. Its not as easy as 1H if the resonances are spread out over a large window.
We also integrate 31P but prefer to use peak height and a calibration against a weighed in standard.
regards
David
David S. B. Grace
Senior Scientist
Life Sciences
GE Healthcare
-----Original Message-----
From: Serkova, Natalie [mailto:Natalie.Serkova_at_ucdenver.edu]
Sent: 4. desember 2013 19:03
To: bbanks_at_pharmacore.com; ammrl_at_ammrl.org
Subject: RE: AMMRL: integration of various nuclei
We do integrate cellular/ tissue metabolites in 31P-NMR - using an MDP (-18 ppm) standard sealed in a capillary.
13C-NMR is more complicated, I believe. Not sure about 19F, but, I think possible.
Natalie
Dr. Natalie J. Serkova
Associate Professor of Anesthesiology, Pharmacology and Radiology Director, UCCC/CTSA Metabolomics and Imaging Core University of Colorado at Denver Anschutz Medical Center Aurora, CO 80045
office phone 303 724 1086
clinical pager 303 266 2910
________________________________________
From: bbanks_at_pharmacore.com [bbanks_at_pharmacore.com]
Sent: Tuesday, December 03, 2013 1:31 PM
To: ammrl_at_ammrl.org
Subject: AMMRL: integration of various nuclei
Folks,
Is it possible to integrate resonances in spectra of nuclei such as 19F, 31P and 13C?
Thanks
Ben
Benjamin Banks, Ph.D.
Associate Director, Analytical R&D
Pharmacore
4180 Mendenhall Oaks Pkwy
High Point, NC 27265
(336) 841-5250 ext 127 phone
(336) 841-5251 FAX
bbanks_at_pharmacore.com
www.pharmacore.com
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Received on Fri Dec 06 2013 - 09:07:23 MST