Perfusion & Flooding

Patrick, Brett E. (patrickb@css.orst.edu)
Wed, 09 Aug 95 17:23:00 PDT

We are interested in using Proton, Pulsed NMR to non-destructively determine
the percentage of oil contained in individual seeds which are fairly small
in size (6-12mg each). A sample of pure oil can be used as a basis. We
would appreciate feedback regarding feasibility, references to single-seed
analysis experts, opportunities to collaborate, and facility availability.
Since our project requires the analysis of approximately 75 seeds, would a
method of autoloading be recommended?

The basis of our interest is a paper by Bruce A. Orman and Robert A.
Schumann, Jr., Nondestructive Single-Kernel Oil Determination of Maize by
Near-Infrared Transmission Spectroscopy (JAOCS, Vol. 69, no. 10, October
1992). Proton, Pulsed NMR was used to make single-kernel oil determinations
for the calibration of a NITS instrument for this paper. The challenge with
our project is that our seeds are much smaller. This seed has an unusually
high concentration of long chain fatty acids.

Thank you for any comments.

Sincerely,

Brett Patrick patrickb@css.orst.edu
Gary Jolliff jolliffg@css.orst.edu
Department of Crop Science, Oregon State University