Please inform those at your institution who might be interested in this
postdoctoral opportunity.
Walther Cancer Research Fellowship - University of Notre Dame
NMR STUDIES OF DNA BINDING PROTEINS
The ideal applicant is an experienced NMR spectroscopist who would like to
learn protein engineering and expression and/or the biophysical chemistry
of DNA recognition. Protein structure and DNA recognition studies are
ongoing using three systems:
So, the product of the sine oculis gene, is a new neurogenic
homeodomain protein from Drosophila which has recently been cloned in
Dr. O'Tousa's lab at Notre Dame. We have overproduced and purified the
63 amino acid homeodomain from E coli with ~20mg/l yield.
Pbx1, is a human proto-oncogene with sequence similarity to So. We are
interested in identifying the structure and function of homologous
sequences outside the classical homeodomain.
Monomeric variants of the lambda Cro Repressor (Mossing and Sauer --
Science 250 1712 (1990)). Assignments are complete (including 15N)
for two variants which differ in the sequence of an engineered beta
hairpin. Second generation structural and DNA-recognition variants are
being constructed for protein stability and DNA complex studies.
The College of Science NMR facility has a Varian Unity 500 MHz spectrometer
and will install a 600 MHz UNITYplus instrument with triple resonance and
pulse field gradient capabilities within the month. Two new UNITYplus 300
MHz spectrometers are also being installed.
Walther Cancer Research Fellowships provide salaries for 2 years at
$30,000/ year, with additional allotments of $7,500/year for supplies and
$1,500/year for travel.
Please contact Mike Mossing for more information. Interested applicants are
asked to submit a curriculum vitae and letters from three references by
Sept. 15, 1994 to: Dr. Joseph E. O'Tousa, Department of Biological
Sciences, University of Notre Dame, Notre Dame Indiana 46556. The
University of Notre Dame is an Affirmative Action/Equal Opportunity
Employer.
Thanks,
Mike Mossing, Department of Biological Sciences, Phone 219/631-8412
University of Notre Dame, Notre Dame, IN 46556-5645 fax 219/631-7413