Can anyone give me some hints for doing a jump-return TOCSY on a Bruker AM
spectrometer? I am trying to reproduce the Bax review sequence in Methods in Enzymology
(V 176, p 165). I am able to invoke this pulse sequence and get a reasonable spectrum,
but the level of water suppression I get is about a factor of 32, not anywhere near
the level of 100 to 200 which is really needed to observe a 1 mm protein.
I would appreciate any comments or suggestions.
Cheers,
Michael J. Jablonsky
NMR Core Facility, U of AL @ B'ham
jabo@mozart.nmrcore.uab.edu