Dear AMMRL-ers
I have a user with some fairly complicated macromolecules with
some intramolecular folding structure which she wants to prove
with NOESY peaks between CH3 protons and some aromatic protons.
The NOESYs she gets do have a ~1% peak between the protons in
question, but seemingly only in the lower half of the NOESY...there may
be very weak upper-half off diagonals in the right place but
in any case it is quite asymmetric. (The phasing is a bit off
but we have not resolved whether that is a processing issue.)
The ROESYs behave similarly.
I have only done nicely behaved smaller molecule NOESYs and find
this asymmetry raises some 'reality' questions about the peak
she does see.
So my query is
1. is this to be believed as a NOESY peak?
2. why does this asymmetry occur?
3. is there literature stuff on this effect/artifact?
Pax
Charlie Dickinson
+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-
+ Charlie Dickinson, PhD -
+ NMR Director -
+ High Field Nuclear Magnetic Resonance Laboratory -
+ Department of Polymer Science and Engineering -
+ University of Massachusetts-Amherst -
+ Amherst MA 01003 USA -
+ PHO (413)577-1428 FAX (413)545-0082 -
+ charlie_at_telemann.pse.umass.edu
Science
Is not what they say it is, so serious
The truth being what you imagine
Not what you see
And not something useful
Or something that pays.
Rebecca Elson
Received on Wed Nov 03 2004 - 12:01:23 MST