Water suppression using presat

Zhang, Weixing (Weixing.Zhang@stjude.org)
Fri, 8 May 1998 12:42:07 -0500

Dear All,

My understanding is that pre-saturation is the original method for water
suppression.
Nowadays, pulsed field gradient is used exclusively for water
suppression. I am using a
presat 2D NOESY experiment to evaluate the performance of a NMR
spectrometer. The
simple pulse sequence is:

relaxation_delay presaturation_delay 90 t1 90 mix 90 Acquire.

I am wondering what is the minimal power (in terms of gamma-H2) and
saturation period
should I use to saturate the water and still get a good spectrum for a
good spectrometer.

Any suggestion is appreciated.

Weixing Zhang, Ph.D.
NMR Manager
St. Jude Children's Research Hospital
Department of Structural Biology
332 North Lauderdale Street
Memphis, TN 38105
Phone: (901)495-3169
Fax: (901)495-3032
http://www.stjude.org