AMMRLers-
We have been working with a small cell bioreactor doing phosphorus NMR to
detect chemical potential changes arising from glucose challenges. We
have done some extracts and found many resonances in the sugar phosphate
regions that do not correspond with what we expected. These are likely
glycolytic intermediates I suspect. I have been looking for a good
reference (book or manuscript) that describes the chemical shift positions
of biologically active phosphorus compounds at pH ranges from 6.9-7.1. I
recall a book in the mid to late 80's that had a chapter dedicated to this
that included every biologically active phosphate compound, its chemical
shift and the pKa if it was an ionizable compound. I do not recall the
reference and have been unable to locate the book, does anyone recall this
chapter?
Any recommendation for a decent paper would also be quite helpful. I have
found references for phosphorus compounds in general, even some web
sources, but specific glycolytic intermediates were not listed.
Thanks
Robert W. Wiseman, Ph.D.
Associate Professor of Physiology and Radiology
Shipping and Mailing Address:
Michigan State University
Department of Physiology
2201 Biomedical and Physical Sciences Building
East Lansing, MI 48824
email: rwiseman_at_msu.edu
alternative email (for files larger than 500 KByte): rww_at_rad.msu.edu
office 3198 BPS Building
517-355-6475 (x-1151)
laboratory 3110 BPS Building
517-355-6475 (x-1342)
Received on Wed Mar 31 2004 - 12:02:20 MST