Hi, Kirk,
You're right. The gNcpmgex_NH experiment doesn't give any useful signal,
even if setting gzlvl5=-6000, gzlvl8=15000, and gzlvl11=29400 as suggested
by Eugene. I modified a pulse sequence based on Nhsqc.c and it worked very
well with Inova 600. The pulse sequence gNcpmgex_X.c and macro gNcpmgex_X
are enclosed. Please check all parameters before typing go. I am not
responsible for any damage.
One more issue with gNcpmgex that is TROSY-version. The experiment works
fine, but it produce doublets in 15N dimension with 600 MHz spectrometer. It
seems it needs applying selective gradients to clean out anti-TROSY
components.
Thanks,
Youlin Xia
University of Minnesota
From: Todd Rappe [mailto:rapp0006_at_umn.edu]
Sent: Wednesday, January 08, 2014 12:41 PM
To: yxia_at_umn.edu
Subject: FW: AMMRL: BioPack gNcpmgex_NH experiment
From: owner-ammrl2_at_webserver2.chem.hawaii.edu
[mailto:owner-ammrl2_at_webserver2.chem.hawaii.edu] On Behalf Of Ryan T McKay
Sent: Monday, January 06, 2014 5:24 PM
To: Kirk Marat
Cc: ammrl_at_ammrl.org
Subject: Re: AMMRL: BioPack gNcpmgex_NH experiment
Hey Kirk,
15N-HSQC using similar pulses works? Check the decoupling sequence as
waveform boards can be problematic. That would be my first suspect.
Best wishes,
Ryan
Sent from mobile device, please forgive typing.
"It's technology married with liberal arts, married with the humanities,
that yields us the result that makes our heart sing." - Steve Jobs
On Jan 6, 2014, at 10:58 AM, Kirk Marat <Kirk.Marat_at_umanitoba.ca> wrote:
Happy New Year everyone.
Has anyone had any experience with the N15 T2 exchange experiment
(gNcpmgex_NH) in BioPack? We can't seem to get it to work or even see any
signal in the first increment, even when the number of CPMG cycles is very
low. Other BioPack sequences seem to work fine, suggesting that the probe
calibration file is fine. Checking on the SPINSIGHTS site, it appears that
at least one other person has had trouble with this experiment (in both VJ
3.2 and 4), but there was no real fix that I could see.
This is an INOVA600 running VnmrJ 3.2A.
Thanks!
Kirk
Kirk Marat, Ph. D. NMR Facility Manager
Dept. of Chemistry, University of Manitoba
Winnipeg, Manitoba
R3T 2N2, Canada
Ph: (204) 474-6259 FAX: (204) 474-7608
Received on Wed Jan 08 2014 - 09:01:57 MST