Hello, everyone
Recently, I have encountered a problem with selective NOE experiment. I want to determine the NOE enhancement of coupled protons by irradiating its coupled partners. However, the NOE spectrum has shown large anti-phase contribution from selective population transfer (SPT effect). Therefore, I couldn't obtain a reliable data about NOE enhancement. I have tried several methods in the Bruker pulse library, for example, selnogp, selnogpzs, selcssfnozs.2. All of them gave me the identical results. I have consulted with the literature, and found there were some possible methods in eliminating this unwanted antiphase cntribution (Fig 8 in J Magn Reson 125, 302-324, 1997). However, this pulse program is not available in Bruker or Joel pulse library. I wonder if anyone have met such problem before and could offer me some hints or solution? My NMR data is processed with TopSpin 3.2pl6.
Thank you very much!
Junfeng XiangNMR Lab, ICCAS, Beijing China
Received on Mon Feb 02 2015 - 17:37:16 MST