Date: Tue, 22 Aug 2023 16:52:13 +0000
Dear All,
Thanks to everyone who has read and replied to my email.
We want to use a CryoProbe to run experiments at different temperatures up to 90 oC (363 K).
Many people think that radiation damping of 80% ethylene glycol is the problem.
Massively detuning the cryoprobe or using deuterated ethylene glycol should give more accurate
results.
Clemens told me that the Bruker part number of 99% ethylene glycol-d6 is Z10629.
Z10629 is currently under development in Germany and it would be about a month for
it to be completed.
The Bruker AU program calctemp has these options.
[cid:image001.png_at_01D9D4EE.D5E3EE90]
[cid:image002.png_at_01D9D4EE.D5E3EE90]
I am curious where Bruker got the equations from, and I would greatly appreciate any information.
With warm regards,
Weixing Zhang (Walter)
Research Operation Manager
Biomolecular NMR Spectroscopy Center
St. Jude Children's Research Hospital
Memphis, TN 38105
USA
> From: main_at_ammrl.groups.io<mailto:main_at_ammrl.groups.io> <main_at_ammrl.groups.io<mailto:main_at_ammrl.groups.io>> On Behalf Of Clemens Anklin via groups.io
> Sent: Friday, August 18, 2023 2:26 PM
> To: main_at_ammrl.groups.io<mailto:main_at_ammrl.groups.io>
> Subject: Re: [AMMRL] 99.8% MeOD vs 80% Ethylene Glycol
Hi Weixing
if you do this on a cryoprobe this will be the expected result. 80% Glycol
gives a massive amount of radiation damping. This leads to different readings
as shown by Stefan Berger in https://doi.org/10.1002/mrc.1941
You can try to run a more accurate measurement by massively detuning the probe.
With best regards
Clemens
On 8/18/2023 3:09 PM, Walter Zhang wrote:
Dear All,
I need to use 80% Ethylene Glycol for high temperature calibration. [cid:image003.png_at_01D9D4EE.D5E3EE90]
Before I do the calibration, I compared it with 99.8% MeOD at 303 K.
When I set temperature to 303 K,
99.8% MeOD gives 303.03 K while 80% Ethylene Glycol gives 300.57 K
The difference between the two calibration samples is 2.46 K.
Is this normal?
Your comments are appreciated!
Best regards,
Weixing Zhang
St. Jude Children's Research Hospital
Memphis, TN 38105
USA
________________________________
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Clemens Anklin Ph.D. Vice President Bruker BioSpin NMR Applications & Training
15 Fortune Drive NEW Phone: 978-313-5NMR(5667) Billerica MA 01821 twitter: _at_canklin web: http://www.bruker.com
e-mail: clemens.anklin_at_bruker.com ==============
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