I have been trying to run a HETCOR expt for about a week now w/o success.
I have calibrated the Carbon 90 degree pulse (ie 9.5 at power=56) and the
decoupler hard pulse
(11 at 56). Using these numbers a dept expt. works fine with excellent
editing - so far as
I can tell.
but hetcor fails. If I set ni=1, and nt=8 (sample in this case was 30%
4-methoxy benzyl alcohol I only get noise (I can see a small spike in the
center of the spectrum). I can get a good C13 spectrum with this sample
with nt=8.
I have tried this both on a 300MHz Varian Mercury and a 400MHz Inova with
similiar results.
both have a 4nuc autoswitching probe, which I am using in the autoswitching
mode.
recompiling the hetcor pulse sequence had no effect.
Both the instrument and myself are fairly new to hetcor. In fact I don't
think this expt has
been run before on either instrument.
I appreciate your help.
Carl
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Carl Bachmann, 214-648-6748
cbachm@biochem.swmed.edu
Department of Biochemistry
UT Southwestern Medical Center
Dallas, TX 75235